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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: J Immunol. 2012 Jan 6;188(4):1970–1980. doi: 10.4049/jimmunol.1102383

Figure 2.

Figure 2

mPGES-1 silencing attenuates MMP-induced MMP-9 expression. [A] RAW264.7 macrophages (24-well plate; 6 × 104/well) transfected with NS or mPGES-1 siRNA were incubated 18 h with DMEM-0.1% LE-BSA (Ctrl) or media containing 10 ng/ml LPS. Total RNA and conditioned media were recovered, and levels of mPGES-1 mRNA and PGE2 were determined utilizing PCR and ELISA, respectively. PGE2 levels are the means (± SD) of 3 experiments. [B] Transfected macrophages (24-well plate; 6 × 104/well) were incubated 18 h with DMEM-0.1% LE-BSA or media containing 50 nM MMP-1, MMP-3 or 10 ng/ml LPS. Total RNA was recovered and levels of MMP-9 mRNA determined. [C] Transfected macrophages (48-well plate; 3.5 × 105/well) were incubated with DMEM-0.1% LE-BSA or media containing MMP-1, MMP-3 or LPS 18 h. Conditioned media and cell lysates were recovered, and levels MMP-9 and GAPDH were determined by Western blot. Data are representative blots and the mean levels (± SD) of MMP-9 mRNA or protein, presented as RDUs, of 4 and 3 experiments, respectively.