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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: J Immunol. 2012 Jan 6;188(4):1970–1980. doi: 10.4049/jimmunol.1102383

Figure 4.

Figure 4

MMP-induced Egr-1 is dependent on TNF-α. [A] RAW264.7 macrophages (12-well plate; 7 × 105/well) were incubated 0 – 18 h in DMEM-0.1% LE-BSA (Ctrl), or media containing 50 nM MMP-1 or MMP-3. [B] In other experiments, macrophages (6-well plate; 2 × 106/well) were pre-incubated for 2 h with non-immune IgG1 (nIgG; 20 μg/ml) or rat monoclonal anti-mouse TNF-α IgG1 (20 μg/ml), followed by 1 h incubation with 50 nM MMP-1 or MMP-3. Total RNA was isolated, and mRNA levels for Egr-1, mPGES-1 and actin were determined by PCR. Data are representative blots and the mean levels (± SD) of target mRNAs, presented as RDUs, of 3 experiments.