Skip to main content
. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: J Immunol. 2012 Jan 6;188(4):1970–1980. doi: 10.4049/jimmunol.1102383

Figure 5.

Figure 5

MMP-induced Egr-1 and mPGES-1 expression are dependent on MAPK kinase. [A] RAW264.7 macrophages (12-well plate; 5 × 105/well) were incubated in DMEM-0.1% LE-BSA (Ctrl) or pre-incubated 30 min in media containing 10 μM U0126 (MAPK kinase inhibitor) followed by the addition of 50 nM MMP-1 or MMP-3, and incubated 1 h. [B] Macrophages (12-well plate; 7 × 105/well) were incubated in DMEM-0.1% LE-BSA or pre-incubated 30 min in media containing 10 μM U0126 followed by the addition of 50 nM MMP-1 or MMP-3, and incubated 18 h. Total RNA was isolated, and mRNA levels for Egr-1, mPGES-1 and actin were determined by PCR. Data are representative blots and the mean levels (± SD) of target mRNAs, presented as RDUs, of 3 experiments.