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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: J Immunol. 2012 Jan 6;188(4):1970–1980. doi: 10.4049/jimmunol.1102383

Figure 7.

Figure 7

EP4 antagonist blocks LPS and MMP-induced mPGES-1 expression. [A] RAW264.7 macrophages (6-well plate; 1 × 106 cells/well) were incubated in DMEM-0.1% LE-BSA alone (Ctrl), or media containing 25 nM MMP-1, MMP-3 or 10 ng/ml LPS. Following 18 h incubation, total RNA was isolated, and mRNA levels for PGE2 receptors (EP1–4) determined by PCR. [B] Macrophages were pre-incubated 30 min in DMEM-0.1% LE-BSA containing 0 – 10 μM EP2 antagonist (AH6809) or EP4 antagonist (AH23848). Following an 18 h incubation with 10 ng/ml LPS, RNA was isolated, and mRNA levels for mPGES-1 and actin were determined by PCR. [C] Macrophages were pre-incubated 30 min in DMEM-0.1% LE-BSA containing 10 μM EP2 or EP4 antagonist. Following an 18 h incubation with media alone (Ctrl), 50 nM MMP-1 or MMP-3, RNA was isolated, and mRNA levels for mPGES-1 and actin were determined by PCR. Data are representative blots and the mean levels (± SD) of mPGES-1mRNA, presented as RDUs, of 3 experiments.