Figure 7.
Increased expression of IL-6 in MPMVEC from wild-type mice exposed to UFPs and/or CSE was attenuated by pre-treatment of cells with p38 inhibitor. 2 × 105 cells were seeded into each well of 6-well plates. After overnight culture, cells were pre-treated with 10 μM SB203580 for 3 h, then treated with UFPs and/or CSE for 12 h. Cells without any treatment were used as controls. Real-time PCR was performed with iQ5 Cycler (Bio-Rad). Data are shown as mean ± SD of three experiments with three replicates in each experiment. * Significant difference as compared with the control, p < 0.05; # Significant difference as compared with UFPs alone or CSE alone treated group, p < 0.05; $ Significant difference as compared with those with UFPs and/or CSE treatment but without SB203580 pre-treatment, p < 0.05.