Fig. 5. Curcumin inhibits the interaction of gastric cancer cells with MDSCs.
The EGFP+ MKN-45 cells and splenic MDSCs were cultured alone or co-cultured in the absence or presence of curcumin. (A) Representative images (magnification 200 ×) were taken 48 hours after curcumin treatment (25 μM) (upper panel). The merged images indicate that the colonies originated from EGFP+ MKN-45 cells (lower panel). Quantification of colonies of cancer cells were shown in (B). The production of IL-6 by MDSCs and its inhibition by curcumin are shown in (C). The data are means ± SD of 3 independent experiments (*p < 0.05, #p < 0.01, compared to DMSO treatment).