Table 1.

Primers for quantitative RT-PCR

Target Gene	Forward Primer (5’-3’)	Conc. (nM)	Reverse Primer (5’-3’)	Conc. (nM)	Expected Length (bp)	Annealing Temp (°C)
β-actin†	CTAAGGCCAACCGTGAAAAGAT	600	CCAGAGGCATACAGGGACAAC	100	103	65
p21	CGAGACACTCAGAGCCACA	300	GCGAAGTCAAAGTTCCACC	300	182	65
p53	ACCTCCACACCTCCACCT	300	AGATACTCAGCATACGATTTC	600	176	60
Bax	AGAGGATGATTGCTGATGTG	100	CGGAGGAAGTCCAGTGTC	600	205	60
Bcl-XL	GGACTGAAGCCCCAGAAGA	600	CAAACTCATCGCCAGCCTC	600	192	60

^†used as reference gene