Figure 1. DETA NONOate added to neuronal culture media for the final 24 hours of Pb2+ exposure spontaneously releases NO and does not affect cell viability.
(A) DETA (10 μM) did not cause a loss in cell viability as assessed by the MTS assay. Cell death was only observed with the excitotoxic concentration (100 μM) of glutamate (Glu). Data are the mean ± SEM and are the result of 4 assays. (B) During the 24 hr treatment period DETA supplementation resulted in significantly elevated levels of NO2 confirming increased NO release. Data are the mean ± SEM and are the result of 3 assays. # = significance from all other treatments, * = significance from treatments without DETA.