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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Oct 13;1823(2):245–254. doi: 10.1016/j.bbamcr.2011.10.002

Fig. 3.

Fig. 3

Effect of protein kinase C and phosphoinositide 3-kinase inhibitors on α1B-adrenergic receptor (α1B-AR) phosphorylation and action. Cells were preincubated in the absence or presence of the following inhibitors for 30 min 1μM LY294002 (+LY), 100 nM wortmannin (+WT), 100 nM staurosporine (+ST) or 1μM bis-indolyl-maleimide I (+BIM). To study (α1B-AR) phosphorylation (upper panel), cells were incubated in the absence of any agent (B, basal) or challenged with 1 μM S1P (all of the remaining conditions) for 15 min. A representative autoradiogram is shown. To study the functional repercussion (lower panel): cells were preincubated with the inhibitors as indicated above and then no agent (NONE) or 1 μM S1P was added. The increase in intracellular calcium induced by 10 μM noradrenaline (NA) was recorded and the maximal effect observed is presented. Means are plotted with vertical lines representing the S.E.M. of 5 to 7 (receptor phosphorylation) or 12 to 15 (intracellular calcium) determinations using different cell preparations. *p < 0.001 vs basal (B, NONE), **p < 0.001 vs S1P.