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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: J Immunol. 2012 Jan 13;188(4):1726–1733. doi: 10.4049/jimmunol.1102879

FIGURE 3.

FIGURE 3

CNS-16 represses human IFN-γ production. A, Transgenic CD4+ cells were cultured under Th1 and Th2 polarizing culture conditions for three days. Human IFN-γ (left) or mouse IFN-γ (right) concentrations were determined in cultures. B, Transgenic CD8+ cells were cultured under Th1 polarizing conditions for three days. IFN-γ was measured in culture by ELSIA. Results are representative of independent replicates. C, Transgenic CD4+ cells were cultured under Th1 conditions for three days and mRNA levels were determined by quantitative PCR. Error bars are standard deviation. D, Transgenic CD8+ T cells were cultured under Th1 polarizing conditions for seven days and restimulated with PMA/Ionomycin. Interferon gamma was determined by intracellular cytokine staining. Quantification of the percentages of human IFN-γ+ cells from three independent replicates is shown. * P < 0.05.