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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: J Immunol. 2012 Jan 13;188(4):1726–1733. doi: 10.4049/jimmunol.1102879

FIGURE 6.

FIGURE 6

CNS+20 is required for IFN-γ production by antigen-stimulated NKT cells. A, Splenocytes were isolated from transgenic mice and stimulated with a mock treatment or with alpha GalCer. Human and mouse IFN-γ concentrations in culture supernatants were determined by ELISA. Results are representative of three independent experiments. Error bars are standard error of the mean. B, Splenocytes from transgenic mice were stimulated with IL-12 and IL-18 and IFN-γ was assayed by intracellular cytokine staining. NKT cells were gated on MHC class II−, CD1d+ populations. * P < 0.05.