Table 1.
Notch ligand expression by CD11c+ DCs of various lymphoid tissuesa
| Tissues | Mice nasally immunized with OVA plus |
% of Total Lymphocytes |
% of CD11c+ DCs | |||
|---|---|---|---|---|---|---|
| CD11c | Dlk 1 | Dlk 4 | Jagged 1 | Jagged 2 | ||
| NALT | Ad-FL | b4.40 ± 0.36** | 17.73 ± 0.82* | 1.21 ± 0.35 | 13.03 ± 0.47* | 9.24 ± 0.86* |
| Ad-Luc | 2.27 ± 0.29 | 13.35 ± 2.08 | 2.49 ± 1.67 | 9.09 ± 2.08 | 7.33 ± 1.83 | |
|
| ||||||
| CLNs | Ad-FL | 3.00 ± 0.08** | 14.94 ± 0.83* | 1.34 ±0.55 | 13.03 ± 1.67** | 7.41 ± 0.96** |
| Ad-Luc | 1.69 ± 0.49 | 10.66 ± 1.08 | 2.37 ± 0.84 | 7.70 ± 1.60 | 5.33 ± 0.84 | |
|
| ||||||
| NPs | Ad-FL | 9.37 ± 0.55** | 25.57 ± 5.04* | 5.94 ± 0.69* | 34.54 ± 4.88* | 36.24 ± 2.14* |
| Ad-Luc | 1.57 ± 0.26 | 10.42 ± 0.97 | 2.13 ± 0.37 | 10.84 ± 1.28 | 7.02 ± 1.91 | |
|
| ||||||
| SMGs | Ad-FL | 13.21 ± 0.96** | 35.33 ± 4.07* | 1.03 ± 0.73 | 24.75 ± 2.24* | 12.52 ± 0.76* |
| Ad-Luc | 3.92 ± 0.24 | 30.56 ± 3.08 | 1.36 ± 0.64 | 10.47 ± 1.99 | 2.47 ± 0.15 | |
|
| ||||||
| Spleen | Ad-FL | 6.15 ± 0.22* | 29.12 ± 2.31* | 1.46 ± 0.59 | 17.95 ± 1.69* | 14.53 ± 0.68* |
| Ad-Luc | 3.31 ± 0.18 | 14.40 ± 0.35 | 1.01 ± 0.46 | 6.95 ± 1.32 | 11.28 ± 1.22 | |
a
Mice were nasally immunized weekly for three consecutive weeks with OVA plus Ad-FL. One week after the last immunization, mononuclear cells from NALT, CLNs, NPs, SMGs and spleen were stained with a combination of the respective mAbs and subjected to flow cytometry analysis by FACSCalibur®.
b
The values shown are the mean ± SEM of three independent experiments. Each experimental group consisted of five mice.
c
*p < 0.05, **p < 0.01 when compared with Ad-Luc group.