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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Clin Immunol. 2011 Oct 19;142(2):160–166. doi: 10.1016/j.clim.2011.10.001

Figure 3. Increased serum IgA, IgA production by splenic B cells and circulating IgA containing immune complexes in WASp-deficient mice.

Figure 3

A: Serum IgA; serum IgA was determined by mouse IgA ELISA quantitation kit. Data were compared between WT control and Was-KO mice at different age groups.

B: In vitro IgA production; splenic B cells were isolated by immunomagnetic isolation kit and the cells were cultured in vitro in the presence of LPS and various cytokines. After 4 d, supernatant samples were harvested and IgA levels determined. Data were compared between WT control and Was-KO mice. Circulating IgA containing immune complexes were also determined by ELISA. Data were compared between WT and Was-KO mice and expressed as OD′ at 405 nm.

C: Serum circulating IgA-containing immune complex (IgA–C3)

D: Serum circulating IgA-containing immune complex (IgA–IgG)