Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2013 Feb 1.

Published in final edited form as: Exp Eye Res. 2011 Oct 25;95(1):48–53. doi: 10.1016/j.exer.2011.10.005

Fig. 2 — (Copyright to ARVO, Koh et al., 2011). Reduced CE damage in corneal buttons (8.5 mm in diameter) trephined from human donor corneoscleral explants that experienced a brief intermittent VIP treatment during their storage. Following the VIP treatment (30 min, 37°C, 10⁻⁸ M) explants were kept in Optisol-GS storage for five additional days before corneal buttons (8.5 mm in diameter) were trephined from them. The buttons were incubated at 37°C for 1 h and then in the presence of CNTF (0.83 nM) for 20–25 h before the CE damage was assessed. (A) Panoramic view of the whole corneal buttons (from one pair of human corneoscleral explants freshly dissected) following alizarin red S staining. (B) Quantification of damaged (alizarin red S-stained) areas in the panoramic photographs of the whole corneal buttons demonstrating the beneficial effect of the VIP treatment. The percentages of CE damage of the whole buttons ( ) were (mean+/−sem) (26.3+/−7.5) and (15.4+/−4.3) %, in buttons from the control and VIP-treated explants, respectively (p= 0.027, N=9 pairs). The damaged CE areas (■) found in buttons from VIP-treated explants were (mean+/−sem) (71.0+/−11.0) % of those in the respective controls (p= 0.016, N=9 pairs). (C) Quantification of the microscopic damage in the corneal endothelium of flat-mounted buttons (corneal endothelium side down) revealed under an inverted microscope (magnification=200X). The percentages of CE damage ( ) were (mean+/−sem) (12.7+/−4.2) and (2.8+/−1.0) %, in buttons from the control and VIP-treated explants, respectively (p= 0.011, N=7 pairs). The damaged CE areas (■) found in buttons from VIP-treated explants were (mean+/−sem) (23.0+/− 4.0) %) of those in the respective controls (p= 6.8X10⁻⁷, N=7 pairs). Both freshly dissected and preserved human donor corneoscleral explants from the eye banks were used.

Inline graphic — (Copyright to ARVO, Koh et al., 2011). Reduced CE damage in corneal buttons (8.5 mm in diameter) trephined from human donor corneoscleral explants that experienced a brief intermittent VIP treatment during their storage. Following the VIP treatment (30 min, 37°C, 10⁻⁸ M) explants were kept in Optisol-GS storage for five additional days before corneal buttons (8.5 mm in diameter) were trephined from them. The buttons were incubated at 37°C for 1 h and then in the presence of CNTF (0.83 nM) for 20–25 h before the CE damage was assessed. (A) Panoramic view of the whole corneal buttons (from one pair of human corneoscleral explants freshly dissected) following alizarin red S staining. (B) Quantification of damaged (alizarin red S-stained) areas in the panoramic photographs of the whole corneal buttons demonstrating the beneficial effect of the VIP treatment. The percentages of CE damage of the whole buttons ( ) were (mean+/−sem) (26.3+/−7.5) and (15.4+/−4.3) %, in buttons from the control and VIP-treated explants, respectively (p= 0.027, N=9 pairs). The damaged CE areas (■) found in buttons from VIP-treated explants were (mean+/−sem) (71.0+/−11.0) % of those in the respective controls (p= 0.016, N=9 pairs). (C) Quantification of the microscopic damage in the corneal endothelium of flat-mounted buttons (corneal endothelium side down) revealed under an inverted microscope (magnification=200X). The percentages of CE damage ( ) were (mean+/−sem) (12.7+/−4.2) and (2.8+/−1.0) %, in buttons from the control and VIP-treated explants, respectively (p= 0.011, N=7 pairs). The damaged CE areas (■) found in buttons from VIP-treated explants were (mean+/−sem) (23.0+/− 4.0) %) of those in the respective controls (p= 6.8X10⁻⁷, N=7 pairs). Both freshly dissected and preserved human donor corneoscleral explants from the eye banks were used.