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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Exp Eye Res. 2011 Oct 25;95(1):48–53. doi: 10.1016/j.exer.2011.10.005

Fig.4.

Fig.4

CE damage in pre-cut cornea reduced by a brief (30 min, 37°C) VIP (10−8 M) treatment of the corneoscleral explants prior to their storage in Optisol-GS. (A) CE damage revealed by intense alizarin red staining of the exposed Descemet’s membrane in areas of denuded corneal endothelium of a flat-mounted control corenoscleral explants under an inverted microscope (Koh et al., 2011). Original magnification=200X. (B) Quantification of the damaged areas in the corneal endothelium of the paired corneoscleral explants. Analysis using Photoshop computer software (Koh et al., 2011; Saad et al., 2008) of 15 CE images taken from the control and VIP-treated corneoscleral explants demonstrated the damaged areas were 4.9+/−1.2 % and 1.1+/−0.4 %, respectively (p=0.002).