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. 2011 Oct 7;107(1):89–100. doi: 10.1007/s11060-011-0729-8

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© The Author(s) 2011

This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

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Fig. 5 — Clinical significance of VIM, CTSD and P4HB in human malignant glioma. a Representative blot images demonstrate high expression levels of VIM, CTSD and P4HB in high-grade (HGG) compared with low-grade gliomas (LGG). Glioblastoma reveals the highest level of expression of the three proteins and is illustrated by asterisk. b Whiskers (min to max) diagram shows the densitometry data from Western blot analysis. Differences are expressed as ratio of the target protein in different grade of glioma to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) density. c VIM, CTSD and P4HB detection by IHC. Representative immunohistochemical staining of VIM, CTSD and P4HB in paraffin-embedded human glioma tissues. Low-grade glioma tissues seen in b, d, f are weakly to moderately stained, while in high-grade tissues, VIM, CTSD and P4HB stainings are strongly intense (A, C, E). Original magnification: ×100; ×400 (insets)