Table 2.

Oligonucleotide primers used in this work

	Primer and description	Sequence
1	lacZp upstream	5′-GGAGACATUCGGGCAGTGAGCGCAACG-3′
2	LacZ downstream	5′-GGGAAAGUGAAAAAGAATAAACCGAACATCCA-3′
3	lacZ upstream (short translation signal)	5′-GGGAAAGUCACAGGAGGGACGTCATGACCATGATTACGGATTCAC-3′
4	lacZ upstream with (long translation signal)	5′-GGAGACATUCATAACAATTTCACACAGGAGGGACGTCATG ACCATGATTACGGATTCAC-3′
5	fnuDIIM upstream with (long translation signal)	5′-GGAGACATUCATAACAATTTCACACAGGAGGGACGTCATG AAAATTAAAGAAAAACGAG-3′
6	fnuDIIM downstream	5′-GGGAAAGUTTAATCTGTTTCTTTTTTAAAATATAG-3′
7	attTn7 upstream	5′-AATCTGTAACGTTCCGGGTTC-3′
8	attTn7 downstream	5′-CATTAATAACGAAGAGATGAC-3′

Note. Primers 1–6 include upstream (5′-GGAGACATU) or downstream (5′-GGGAAAGU) sequences needed for subsequent USER cloning. The 3′-end of primer 1 anneals 133 bp upstream of lacZ ATG; the 3′-end of primer 2 anneals 80 bp downstream of lacZ stop codon TAA; primers 3 and 4 to 17 nucleotides of lacZ open reading frame beginning with ATG; primer 5 anneals to 22 nucleotides of fnuDIIM gene starting at ATG; primer 6 anneals to 26 nucleotides downstream of fnuDIIM gene ending at stop codon TAA. Translation initiation signals are italicised. Primers 7 and 8 flank the glmS terminator, into which the mini-Tn7 (mTn7) inserts.