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. 2011 Sep 29;40(3):1251–1266. doi: 10.1093/nar/gkr791

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Overexpression of SMG-5-dCT or SMG-6-dCT causes the accumulation of phospho-Upf1 complexes containing different components. (A) Schematic representation of SMG-5dCT and SMG-6dCT mutants. The 14-3-3-like domain and PIN domain are depicted in gray boxes. SMG-5dCT and SMG-6dCT have deletions of the C-terminal 23 or 11 amino acids, respectively. (B) Overexpression of SMG-5 or SMG-6 mutants results in the accumulation of Upf1 phosphorylated at T28 and S1078/S1096. Cells were transfected with the SMG-5 or SMG-6 plasmids indicated above the blot. Total cell extracts were probed with the antibodies indicated. (C) HeLa TetOff cells were transfected with the indicated plasmids. The cell extracts were immunoprecipitated with anti-Upf1 (5C3) antibody in the presence of RNaseA. (D) Schematic presentation of Upf1 complexes following overexpression of SMG-5-dCT or SMG-6-dCT.