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. 2011 Oct 3;40(3):1065–1076. doi: 10.1093/nar/gkr805

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Analysis of ligase activity and complex formation with UBZ mutants of RAD18. (A) Purified complexes (3.7 pmol) were analyzed by SDS–PAGE followed by staining with CBB. I, RAD6A–(^HisRAD18–^FLAGRAD18); II, RAD6A–(^HisRAD18^C207F–^FLAGRAD18^C207F); III, RAD6A–(^HisRAD18^D221A–^FLAGRAD18^D221A). Structures are represented schematically, UBZ domains with a mutation being shown in white boxes. (B) Ligase activity of the respective RAD6A–RAD18 complexes. Increasing amounts of the complexes (0.5, 1 and 2 pmol) shown in (A) were subjected to standard assays.