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. 2011 Oct 5;40(3):1148–1159. doi: 10.1093/nar/gkr813

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — In vivo stability of TrfA variants in wild-type and protease-deficient strains. The stability of TrfA protein was analyzed after inhibition of translation by tetracycline in TrfA-overproducing bacteria. Cells of E. coli strains C600, C600 clpP⁻ and C600 lon⁻ harboring plasmids for overproduction of wt TrfA, TrfA G254D/S267L or TrfA P151S/G254D/S267L were used. Samples taken from the cultures at indicated time points after the addition of tetracycline were analyzed for TrfA presence by SDS–PAGE followed by immunoblot with anti-TrfA antibodies.