Figure 7.

Formation of Lon–TrfA–DNA nucleoprotein complex. Formation of nucleoprotein complex involving Lon and TrfA in real-time was studied using SPR on a sensor chip with immobilized pUC19 DNA fragments (frag2) on its surface. Buffer used for the analysis was HBS-EP supplemented with 10 mM magnesium acetate and 2 mM ATP. (A) Lon (200 nM), TrfA P151S/G254D/S267L (80 nM) or both were pre-incubated for 2 min in HBS-EP and subsequently injected onto sensor chip. (B) Injection of 200 nM Lon (solid line) or buffer (dashed line) onto sensor chip was rapidly followed by injection of 80 nM TrfA P151S/G254D/S267L.