Table 1.
Escherichia coli C600 strain | Plasmid loss rate (% per generation)a |
---|---|
Wild-typeb | 0.12 ± 0.01 |
clpP−b | 0.24 ± 0.02 |
lon−b | 0.33 ± 0.01 |
Wild-type (pBAD24)c | 1.46 ± 0.05 |
Wild-type (pBADLon)c | 3.23 ± 0.94 |
aGiven values are means from three independent repeats of each experiment.
bStability of pRR10 minireplicon was analyzed in C600 and its mutant derivatives cultured in antibiotic-free medium
cStability of pTJS42 minireplicon was analyzed in wild-type C600 strain cells carrying either pBAD24 or pBADLon cultured in ampicillin-containing medium.