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. 2011 Oct 3;40(3):981–995. doi: 10.1093/nar/gkr818

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — HBP1 was acetylated within the cells. (A) Exogenous HBP1 was acetylated within the cells. 2BS cells transfected with HBP1(HA-tagged) were treated with TSA at 1 μM for 18 h. IP assay was carried out by using HA antibody and followed by western blot with acetyl-lysine antibody (left). IP assay was carried out by using acetyl-lysine antibody and followed by western blot with HA antibody (right). (B) Endogenous HBP1 was acetylated within the cells. 2BS cells were treated with TSA at 1 μM for 18 h. IP assay was carried out by using HBP1 antibody and followed by western blot with acetyl-lysine antibody.