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. 2011 Oct 5;40(3):1009–1020. doi: 10.1093/nar/gkr830

Figure 2.

Figure 2.

DNA-binding motif assays for Ms6564. (A) DNaseI footprinting experiments. The assay of the protection of Ms6564 promoter DNA was performed against DNaseI digestion by increasing the amount of Ms6564 (lanes 1–4). The ladders are shown and the corresponding nucleotide sequence is listed (lanes 5–8). The protected regions on the coding strand (left panel) and non-coding strand (right panel) are indicated by a black bar. (B) Sequence and structural characteristics of the protected Ms6564 promoter region. The regions protected by Ms6564 are shown with underlines and the box highlights the 19 bp sequences containing the invert repeat (IR) with 3 bp separations. The translation start codon of Ms6564 is indicated in bold. (C) EMSA assays for the DNA-binding activity of Ms6564 on the DNA substrates with (lanes 1–4) or without the IR sequence (lanes 5–8). Either DNA substrate was co-incubated with 0.25–1 µM Ms6564 protein.