Figure 7.

ATPase and helicase activity of the PS1 and the EXT mutants. (A) Schematic view of the location of the mutations in the BcMCM model and their implications in the different proposed extrusion mechanisms variants to unwind DNA (16). Each model threads the DNA strands in a different way through the helicase central and hypothetical lateral channels, which are depicted as a shaded inner surface. (B) ATPase (upper panel) and helicase (lower panel) activity of the PS1 and EXT BcMCM mutants. The histograms show the average of three experiments and the error bars indicate the SEM. A representative gel displaying the helicase experiment is depicted in the right side. Both assays were performed as in Figure 3.