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. 2011 Oct 13;40(3):1131–1147. doi: 10.1093/nar/gkr834

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Protease activity of GP63 in six Leishmania species. (A) Western blot using monoclonal antibody targetting GP63 shows the quantity of this protease in each sample. (B) Gelatin zymography assay determining the protease activity of GP63. No signal was observed for L. tarentolae, suggesting the absence of GP63 activity in this species. Lane 1, L. mexicana; lane 2, L. major; lane 3, L. donovani; lane 4, L. infantum; lane 5, L. amazonensis; and lane 6, L. tarentolae.