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. 2011 Oct 13;40(3):1131–1147. doi: 10.1093/nar/gkr834

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Southern blot hybridization of genes whose copy number varies between L. tarentolae and the other Leishmania pathogenic species. Total genomic DNA of Leishmania isolates was digested with XhoI, run on agarose gels, blotted and hybridized with a combination of PCR-specific probes derived from each species (see Supplementary Table S2 for primer sequences and probe details). (A) Delta-amastin; (B) Proto-delta-amastin (shared by the four species) as a control; (C) Phosphoglycan β 1,3 galactosyltransferase; (D) Phosphoglycan β 1,2 arabinosyltransferase; (E) GP63; and (F) Surface antigen protein PSA31C. DNA loading was estimated by hybridizing the same blot with the single copy gene PTR1 (shown in the lower portion of each panel). Lanes 1, L. tarentolae Parrott-TarII; lane 2, L. tarentolae S125; lane 3, L. major Friedlin; lane 4, L. infantum JPCM5; lane 5, L. braziliensis WHOM/BR/75/M2904.