Figure 2.

Distribution of RNAPII along the lys2-lacZ transcription units of plasmids pTGlyla and pTGlylaTER. ChIP analyses in the wild-type strain (HRN1-4A) carrying the plasmids pTGlylaTER (A) or TGlyla (B). Schemes of the gene and the PCR-amplified fragments are shown. DNA ratios in regions 1–4 were calculated from the amounts obtained for these regions relative to the amounts of the intergenic region. ChIPs were performed from three independent cultures, and quantitative PCRs were repeated three times for each culture. SDs are indicated as error bars.