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. 2011 Oct 13;40(3):1050–1064. doi: 10.1093/nar/gkr840

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Northern analyses of convergent transcription in the lys2-CYCt-lacZ and lys2-lacZ fusion constructs. (A) Northern analyses of lys2-CYCt-lacZ mRNAs driven from the tet and GAL1 promoters in wild-type (HRN1-4A), top1Δ (TOHR-11A), top2-1 (TORH-6A) and top1Δ top2-1 (TOHR-13C) strains. The 3-Kb XbaI-EcoRV lacZ internal fragment were used as DNA probe. (B) Northern analyses of lys2-lacZ mRNAs driven from the P_tet and P_GAL1 promoters. Other details as described for Figure 3.