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. 2011 Oct 13;40(3):1050–1064. doi: 10.1093/nar/gkr840

Figure 8.

Figure 8.

Recombination analyses of wild-type, top1Δ, top2-1 and top1Δ top2-1 strains in DNA substrates undergoing converging transcription. (A) Recombination frequencies were determined in wild-type (HRN1-4A), top1Δ (TOHR-11A), top2-1 (TORH-6A) and top1Δ top2-1 (TOHR-13C) strains transformed with plasmid pLlyla carrying the direct-repeat system L-Llyla in which transcription is under the PLEU2 promoter. (B) Recombination frequencies in wild-type (HRN1-4A), top1Δ (TOHR-11A), top2-1 (TORH-6A) and top1Δ top2-1 (TOHR-13C) strains transformed with plasmid pLlylaTER carrying the direct-repeat system L-LlylaTER. For more details see ‘Materials and Methods’ section.