Figure 4.

Target preference of miR-96 and miR-182 depends on presence of a 8-mer site. (A) Schematic representation of the indicated mRNAs with their miR-96/182 sites. The type of site as defined by Targetscan is as indicated (see the legend at the bottom-right). (B) HuH7 cells were transfected with small RNAs as indicated. Three days later, the amounts of FN1, FOXO1 and GAPDH proteins were measured. Representative of three independent experiments. (C) miRNA:target 3′-UTR pairings as predicted by miRWalk using the indicated programs.