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Cleavage and reassembly of synthetic gene product during ECR. Synthetic rfp gene (lane 1) was incubated with Surveyor nuclease for 20 min (lane 2) and 60 min (lane 3) at 42°C. The cleavage reaction products (lanes 2 and 3) were then re-assembled by OE-PCR into full-length gene products (lanes 4 and 5, respectively, marked by arrow). The reaction products were analyzed by agarose gel electrophoresis with DNA molecular weight marker (lane M).
