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. 2011 Nov 29;40(3):e21. doi: 10.1093/nar/gkr908

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Insertion of DHSV at Atm causes DNA damage sensitivity. (A) Structure of mutant Atm locus. (B) Triple primer PCR genotyping using primers a, b and c shown in (A). Clones 2, 3 and 5 lack the wild-type band, consistent with being homozygous mutants. (C) RT–PCR showing loss of Atm transcript. Internal primers to LacZ were included as a positive control. (D) Atm mutants are hypersensitive to adriamycin. Mean surviving fraction (colonies) is plotted. NN5, n =2; Atm⁻^/⁻, n = 3. Error bars show SEM.