Figure 6.

Robust disruption of transcription in homozygous mutants. (A) PCR genotyping of DHSV insertion in Ddef2 using primers for mutant (Junction, left) and wild-type alleles. All double-resistant subclones are homozygous mutants. (B) RT–PCR showing amplification of fusion transcript (left) and loss of wild-type transcript (right) in double-resistant subclones. (C and D) Insertions of the TNN vector also disrupt transcription. PCR and RT–PCR genotyping is shown for insertions in Dym (C) and Arrb2 (D). Asterisks indicate homozygous mutant subclones, all of which have lost wild-type transcript expression. Minus indicates no template control, Actb is an internal control.