Figure 5.
pre-RC and BrdU-incorporating sites detected in the wild-type and rif1Δ cells by ChIP–chip assays. (A) Cells were harvested at 60 min after release from M-phase arrest in the presence of HU as described in the legend for Figure 3. The number of Mcm4-BSs in hsk1-89 cells (at 30°C) and those of BrdU-incorporation sites in wild-type and rif1Δ cells are presented. (B) The numbers of origins up-regulated or down-regulated in rif1Δ cells are indicated. The numbers of up-regulated or down-regulated origins on each chromosome are also shown. (C) The identified ori were classified into four categories according to their overlap with BrdU domains: EE (“early to early”; early in wild-type and early in rif1Δ), LL (“late to late”), EL (“early to late”) and LE (“late to early”) The numbers of EE, LL, EL, and LE are presented.