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. Author manuscript; available in PMC: 2012 Feb 7.
Published in final edited form as: J Cell Biochem. 2010 Apr 1;109(5):967–974. doi: 10.1002/jcb.22475

Figure 2. POH1 reduces ubiquitination of Mitf.

Figure 2

(A) Representative Western blot of Mitf immunoprecipitates from RAW 264.7 c4 cells stimulated with M-CSF (10 ng/mL) or M-CSF (10 ng/mL) and RANKL (60 ng/mL) for 2 days and immunoblotted against ubiquitin (Cell Signaling). (B) Representative Western blot of 293T cells transiently transfected with FLAG-Mitf, HA-ubiquitin and wild type or mutant POH1. Lysates were collected 24 hours following transfection and analyzed by Western blotting with a HA-tag antibody. The lower panel was immunoblotted with a FLAG antibody.