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. Author manuscript; available in PMC: 2012 Feb 7.
Published in final edited form as: J Cell Biochem. 2010 Apr 1;109(5):967–974. doi: 10.1002/jcb.22475

Figure 3. POH1 increases Mitf’s ability to activate transcription.

Figure 3

(A) NIH 3T3 cells were transiently transfected with TRAP luciferase reporter construct and/or Mitf and POH1. *p≤0.05 vs. Acp5-luc+Mitf. (B) NIH 3T3 cells were transiently transfected with 5X Gal4-TK-luciferase reporter construct and/or pMI-Mitf and full length POH1. pMI-MITF encodes amino terminus (amino acids 1-185) of Mitf fused in frame to the Gal4 DNA binding domain. Luciferase reporter activity is presented as relative luciferase units (RLU) and the results of three experiments each performed in duplicate are presented. *p≤0.05 vs.gal4-TK-luc+Mitf. (C) Real time RT-PCR analysis of osteoclasts treated with or without 25 µM MG132 6 hours before harvest for expression of Acp5 (encoding TRACP) or cathepsin K RNA. ***p≤0.0005 vs. M-CSF treatment and *p≤0.05 vs. RANKL treatment for Ctsk and *p≤0.05 vs. M-CSF treatment and NS, not significant vs. RANKL treatment for Acp5.