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. Author manuscript; available in PMC: 2012 Feb 7.
Published in final edited form as: J Cell Biochem. 2010 Apr 1;109(5):967–974. doi: 10.1002/jcb.22475

Figure 4. Amino terminus of POH1 is sufficient to interact with Mitf.

Figure 4

(A) Myc-tagged POH1 constructs and FLAG-tagged Mitf were expressed in 293T cells and complexes from whole cell lysates were immunoprecipitated with FLAG antibody and analyzed by Western blot with the indicated antibodies. (B) NIH 3T3 cells were transiently transfected with 5X Gal4-TK-luciferase reporter construct and/or pMI-Mitf and amino terminus of POH1 (amino acids 1-180). Luciferase reporter activity is presented as relative luciferase units (RLU) and the results of three experiments each performed in duplicate are presented. **p≤0.005 vs. Gal4-TK-luc+pMI-Mitf.