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. Author manuscript; available in PMC: 2012 Feb 7.
Published in final edited form as: J Cell Biochem. 2010 Apr 1;109(5):967–974. doi: 10.1002/jcb.22475

Figure 5. MPN+ domain of POH1 is required for regulation of Mitf’s activity.

Figure 5

(A) NIH 3T3 cells were transiently transfected with 5X Gal4-TK-luciferase reporter construct and/or pMI-Mitf POH1 point mutants in the MPN+/JAMM motif. Luciferase reporter activity is presented as relative luciferase units (RLU) and the results of three experiments each performed in duplicate are presented. *p≤ 0.05 vs. Gal4-TK-luc+pMI-Mitf. (B) Real time RT-PCR from RAW 264.7 c4 cells that were either mock transfected, transiently transfected with full length POH1 or full length POH1 containing the point mutant C120S. Cells were stimulated with M-CSF (10 ng/mL) or M-CSF and RANKL (60 ng/mL) for 5 days. **p≤0.005 vs. M-CSF treatment.