Figure 5.

Growth effect of brucine immuno-nanoparticles on liver cancer cells. Liver cancer cells shrank and pseudopodia disappeared at a brucine concentration of 1.0 μg/mL in brucine immuno-nanoparticles for 72 hours in vitro (A). The number of liver cancer cells dropped, and liver cancer cells were sparse and round at a brucine concentration of 40 μg/mL in brucine immuno-nanoparticles for 72 hours in vitro (B). Liver cancer cells showed cytoplasm “bubble” phenomena and low adhesion capacity, and more cell debris could be found at a brucine concentration of 160 μg/mL in brucine immuno-nanoparticles for 72 hours in vitro (C) (200× magnification).