Figure 2.

Effect of NIR815 dye on human mesenchymal stem cells. (A) Flow cytometry analysis of unlabeled and NIR815 human mesenchymal stem cells, 0 and 20 hours after staining procedures. (B) Cell proliferation, cumulative number of human mesenchymal stem cells at different days in vitro following labeling with NIR815 dye. (C) Cell survival, with cell viability examined by MTS assay at different time points as indicated below the graph.

Note: Data are presented as the mean ± standard deviation from three replicates of three different experiments.

Abbreviations: OD, optical density; PI, propidium iodide; hMSCs, human mesenchymal stem cells; DIV, days in vitro.