Skip to main content
. 2012 Jan 31;7:435–447. doi: 10.2147/IJN.S27537

Figure 4.

Figure 4

Dye transfer. Cells were double-labeled with Hoechst and CellVue® NIR815, their conditioned media taken up at various time points (24 and 72 hours) and used to assess possible leakage of either dyes. (A) Detection of Hoechst staining (blue) in CD90-positive hMSCs (red). CTR+: human mesenchymal stem cells directly labeled with the NIR815 dye (positive control); CM72: CD90 human mesenchymal stem cells incubated with conditioned media obtained 72 hours after NIR815 labeling. Scale bar 10 μm. (B) Detection of NIR185 signal in CD90-positive human mesenchymal stem cells. CD90 signal is shown in red, CellVue signal is shown in green.

Abbreviations: CTR−, CD90-positive human mesenchymal stem cells in the absence of NIR815 staining; CTR+, CD90 human mesenchymal stem cells directly labeled with the NIR815 dye; CM24, CD90 human mesenchymal stem cells incubated with conditioned media obtained 24 hours after near-infrared labeling; CM72, CD90 human mesenchymal stem cells incubated with conditioned media obtained 72 hours after NIR815 labeling.