Figure 3.

Formation of monolayers on PC Transwells^® with 3 μm pores was measured using TEER and Na-Flu transport. Monolayers (insert plus cells) were compared with blank inserts (insert alone) on days 3, 4 and 5 PS and TEER values were blank-corrected. (A) Mean TEER values ± SD (n = 3). (B) Concentration of Na-Flu detected in basal chamber 3 hours after apical application. Values are corrected for background fluorescence and expressed as mean ± SD (n = 3).

Abbreviations: PC, polycarbonate; PS, post-seeding; SD, standard deviation; TEER, transepithelial electrical resistance.