Table 2.
Operating Parameter | Specifications and Conditions |
---|---|
HPLC System | Shimadzu SCL-6B with Shimzdzu SPD-6AV UV/VIS detector at 360 nm |
Analytical column | Shim-Pack CLC-ODS (M) 4.6 mm × 150 mm with a C18 guard column |
Mobile phase | A: acetonitrile 100 (V), B: water/acetonitrile/tetrahydrofuran 50/45/5 (v/v) |
Gradient elution | 100% B for 5 min, and then 60:40% A:B for 15 min, finally 100% A for 5 min |
Flow rate and injection volume | 1.0 mL/min and 20 μL injection |