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(a) Schematic representation of the modular RevNES-biosensor. (b) In living HeLa cells transiently expressing the RevNES-biosensor, the protein localized predominantly to the cytoplasm, whereas treatment with LMB resulted in its nuclear accumulation. Scale bar, 10 μm. (c) Schematic illustration of the masks used to mark the nucleus and the cytoplasm in order to define the cytoplasmic to nuclear translocation of the autofluorescent biosensor (details see text).
