Figure 2.

PLZF mRNA levels during development and in restraint-stressed adults. a, Quantitative RT-PCR of cochleae (organ of Corti and spiral ganglia) indicated that PLZF mRNA levels increased until P30, and declined rapidly thereafter. b, Restraint stress increased cochlear PLZF mRNA in the adult (P90). Levels increased during the period of restraint (shaded bar), peaking at 8 h (p < 0.0001 when compared with time 0 by unpaired Student's t test) and returned toward normal even during continued restraint. The time course of PLZF changes in the brain were similar to those in the cochlea (p < 0.001 at 6 h). Plotted are means ± SE. Each point in a represents data from 3–4 mice. Each point in b represents four to six 10- to 12-week-old CB mice, except for time point 0 (8 mice).