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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: J Liq Chromatogr Relat Technol. 2011 Dec 1;34(20):2505–2515. doi: 10.1080/10826076.2011.591030

Fig. 2.

Fig. 2

HSCCC chromatograms for optimization suitable solvent systems for separation of the targets from the crude extract of E. adenophorum Spreng. Solvent system: a. ethyl acetate–methanol–water at volume ratio of (100: 1: 100, v/v); b. ethyl acetate–methanol–water at volume ratio of (50: 1: 50, v/v); c. ethyl acetate–methanol–water at volume ratio of (10: 1: 10, v/v); d. ethyl acetate–methanol–water at volume ratio of (5: 1: 5, v/v). Stationary phase: organic phase; mobile phase: lower aqueous phase. Flow-rate: 2.0 ml/min; revolution speed: 800 rpm; sample: 378.5 mg dissolved in 10 ml lower phase. Retentions of the stationary phase of these four solvent systems were 69.6%, 69.6%, 65.2% and 56.5%, respectively.