Fig. 2.

Her proteins form DNA-binding and non-DNA-binding dimers in vivo. (A-F) Representative images of Her1 (A), Her7 (B), Hes6 (C), Her1+Her7 (D), Her1+Hes6 (E) and Her7+Hes6 (F) proteins. (G) A chart summarizing the dimers that bind DNA in vitro (denoted by a +) and the dimers that form in vivo (denoted by a green box). All the dimers observed in vitro, except Her12/Her12, were observed in vivo in addition to the presence of four novel dimers. (+/–) Her11 weakly binds DNA, and although we have observed binding in multiple independent experiments, we frequently did not detect the faint gel shift.