Figure 5.
Therapeutic vaccination using Ad5-KH. BALB/c mice were infected with Leishmania donovani and at day 21 after infection, immunized in the footpad with 109 pfu Ad5-KH. A, Parasite burden was evaluated at day 31 from Giemsa-stained impression smears and is expressed in Leishman Donovan Units (LDU; [13]). Data are pooled from three independent experiments (20–23 mice per group). Mean ± standard error of the mean parasite burden is indicated. Significant differences in parasite load as determined by ANOVA are shown in horizontal bars. B, Percentage inhibition of parasite growth at day 31 was calculated for mice vaccinated with Ad5-KH and Ad5-GFP, according to the formula: (LDU d31 unvaccinated - LDU d21) - (LDU d31 vaccinated - LDU d21)/(LDU d31 unvaccinated - LDU d21) × 100 [22]. Data were analysed by Student’s t test.