Figure 6.

Immune responses induced by vaccination with Ad5-KH in Leishmania donovani–infected mice. A, CD8⁺ T cell responses evaluated by ELISPOT in mice vaccinated with Ad5-GFP (top) or Ad5-KH (bottom). B and C, Peptide-specific IFNγ responses to KMP11_HYEKFERMI and HASPB_GGPKEGENL were evaluated by flow cytometry and presented as frequency of responding CD8⁺ T cells (B) and as absolute number of peptide specific CD8⁺ T cells per spleen (C). Values of individual mice are plotted. Data are derived from one of the 3 experiments included in Figure 5 (in which inhibition of parasite growth induced by Ad5-KH was 96% ± 6% and by Ad5-GFP was 56% ± 10%; n = 8; P < .01). D–G. IgG1 (D and E) and IgG2a (F and G) antibody responses to KMP11 (D and F) and HASPB (E and G) were determined by ELISA using serum from unvaccinated infected mice (circles) and infected mice vaccinated with Ad5-GFP (squares) or Ad5-KH (triangles). Data represent mean ± standard error of the mean derived from 6–8 mice per group from one experiment.